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EI is autophosphorylated on a histidine residue by PEP in the presence of Mg^sup 2+^, and the phosphoryl group is then transferred to HPr (1)
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These changes in the thermodynamical parameters point to subtle conformational variations in the packing of some residues
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In addition, all thermal transitions in NDSB solutions exhibit also the missing reversibility as observed for pure buffer solutions
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The displacement error increases with magnetic field strength and decreases with rf pulse bandwidth
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For both Cu and Ni substrates, R flux showed the worst performance
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Consequently, the k^sub ex^ values calculated in this way (Eqs
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In comparison, the ThT fluorescence peak in a water-glycerol mixture remains constant at 492 nm
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Despite limited accuracy in the particle size determination and limited resolution, this system facilitates observation of lipoproteins, lipid-free and lipid-poor proteins on the same gel and is widely used for lipoprotein analysis (Davidson et al
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METHODSThree simulations of 10 ns at 300 K, 1 atm, were carried out for three different systems in explicit solvent: free HIV-1 gp120 with the truncated LAI strain sequence (SWISS-PROT accession number P03377; 346 residues and 29,047 water molecules), free CD4 (99 residues of the D1 domain and 7768 water molecules), and their complex (445 residues and 30,242 water molecules)
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An upper limit to the standard error at equilibrium can then be estimated by fitting the block average standard errors as a function of increasing trajectory block sizes to a single-exponential function
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Taking a 1 wt% solution in a theta solvent above the theta temperature and cooling it rapidly achieved the phase separation
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To confirm these assignments, we examined a destabilized 4WJ ribozyme variant, in which G^sub 11^ is replaced by inosine (I)
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