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5 B clearly indicates that the change of the band at 1628 cm^sup -1^ was the dominant event at temperatures below 66°C, whereas the asynchronous plot was dominated by noise and no crosspeaks could be identified at a similar position in the synchronous plot
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The protein was eventually cluted in 0
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However, quenching experiments and proteolysis patterns suggest conformational changes in the presence of PEP; furthermore, the thermal stability of EI^sup SC^ was modified depending on the effector added
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The structure of the modeled dimer nicely explained why the solvent accessibility of the Tip^sup 461^ was reduced as the concentration of EI^sup sc^ increased (see before)
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Although the fluorescence intensity signal exhibits this proportionality to the population of the microstates (i
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The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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To look at the generality of the phenomenon, ANS binding experiments were carried out on seven new proteins, namely, monomers (bovine serum albumin (BSA), α-amylase, and β-galactosidase (β-gal)), a dimer (alcohol dehydrogenase from horse liver (LADH)), and tetramers (alcohol dehydrogenase from yeast (YADH), lactic dehydrogenase (LDH), and aldolase (ALD))
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The protein bands were visualized through Coomassie R-250 staining
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By comparing the volume change of PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), we can estimate the volume of activation of PLCβ^sub 2^ by Gβγ-subunits
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Fluorescence measurements and analysisFluorescence measurements were performed on an ISS spectrofluorometer (Champaign, IL)
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Similar delays have been reported for the Argentine hake (Merluccius hubbsi) (Pajaro and Macchi (7); Machinandiarena et al
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van Gunsteren, and J
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