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Estefanía Hurtado-Gómez,* Gregorio Fernández-Ballester,* Harald Nothaft,[dagger] Javier Gómez,* Fritz Titgemeyer,[dagger] and José Luis Neira*[double dagger] * Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Alicante, Spain; [dagger] Lehrstuhl für Mikrobiologies, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany; and [double dagger] Biocomputation and Complex Systems Physics Institute, Zaragoza, Spain Submitted October 31, 2005, and accepted for publication March 7, 2006
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To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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