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. An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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. At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range
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. PyMOL molecular graphics system on World Wide Web URL: http://www
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. The Cu-Sn reaction is selective15 and therefore a very rough scalloplike Cu^sub 6^Sn^sub 5^ IMC layer was detected
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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. With increasing attraction there is the potential to form particle networks or gels (68-70)
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. Charge neutralization of DNA is a driving force of DNA compaction
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. 5 mg/ml protein concentration was stored in the dark at 4°C and was used in four weeks, during which no protein degradation was detected
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. Partitioning the configurational space in this way, and by providing an attractive "gutter", relying on local forces alone, to connect one diffusive subspace to the next, creates the advantageous funnel landscape when the high-dimensional structure is projected into a one- or two-dimensional space
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. As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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. Each emerges from the high-dimensional hypergutter picture when a different projection into a low-dimensional space is applied
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