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By using a combination of fluorescence and circular dichroism spectroscopies, thiol group titration, and size-exclusion chromatography we have detected the existence of partially folded intermediate states in the unfolding of GR
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9 C shows anisotropy decay from TTR aggregation reactions collected after 1, 11, 33, and 125 min of A-state initiation
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Fluorescent molecular rotors: a new class of probes for tubulin structure and assembly
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Characterization of CPM-PLCβ-Gβγ associations at atmospheric pressureIn Fig
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The thermodynamically irreversible step in disk denaturation is protein unfolding/dissociation and disk fusion that compensates for the reduction in the particle surface, leading to a high kinetic barrier for the disk denaturation (Gursky et al
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Probing the mechanism of insulin fibril formation with insulin mutants
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In this procedure, the 50 µs data was first rebinned in 500-µs time bins, and rebinned data points lying above a threshold of 40 counts were identified (i
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Tissue sections were incubated in an antigen retrieval solution (citrate buffer) (BioTek Solutions, Santa Barbara, Calif) in a microwave oven and stained on a Tech Mate 1000 automated immunostainer using an avidin/biotin complex staining procedure (BioTek Solutions)
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Fong R, Kissmeyer-Nielsen F
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