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Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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We find that residues with significant exchange broadening (Table 1) group in a model structure of the CaM:SEF2-1mp complex into three regions: i), the CaM dimerization interface, i
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