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These folding intermediates exhibited flexible tertiary structures and nativelike secondary structures
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A significant intensity decrease was observed for most IR bands from native secondary structures, accompanied with the exposure of most Trp residues to water and the formation of aggregates during the I [arrow right] A transition
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The HuBChE samples were assayed before and after neutron scattering experiments by the Ellman procedure (36), with butyrylthiocholine iodide (1 mM) as the substrate
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6 a shows a representative example of the morphological variability in insulin fibrillar aggregates grown for 10 min at 70°C
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Financial support from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie and the COST D30 action "High Pressure Tuning of Biochemical Processes: Protein Dynamics and Aggregation" is gratefully acknowledged
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Mark-recapture and telemetry studies could be used to investigate the spatial dynamics of reproductive activity of this species in the Argentine Sea
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ResultsOvarian developmentBlack rockfish off Oregon exhibited group-synchronous oocyte development; and females extruded only one brood of larvae per year (Fig
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After removing the PBS, 200 [micro]L of the hybridoma culture supernatant solution was added to the wells and incubated at room temperature for 30 minutes
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