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Firstly, those residues making van der Waals clashes were selected and lilted with "Quick and Dirty" algorithms: and secondly, models were energy minimized (100 steps of steepest descent and 100 conjugate gradients, with a cut-off of 10 [Angstrom] for nonhonded interactions) with Insight II (Biosym/MSI) addiction pain severe ultram .
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