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The original IR or fluorescence data were normalised by the corresponding intensity of the spectra recorded al 30°C
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Details of these tests for convergence of the simulations and the sample size are presented in the Appendix
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The distortion of the native structure inferred from ANS binding was found to draw a parallel with the extent of irreversible denaturation by freeze-thawing, suggesting that these altered conformations play a direct role on freeze damage
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We first analyze the latter class and demonstrate that additional binding of ANS in ice is due to the creation of new binding sites rather than to a more complete saturation of preexisting weak sites after the large freeze-induced concentration of the ligand
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15 mN for eutectic Sn-Pb solder and 1
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Furthermore, the CD melting curves shift to lower temperatures upon reduction in the heating rate from 1
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The onset of this transition at various scan rates is observed at T^sub R^ = 32-34°C, similar to T^sub R^ interred from the CD data
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