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Between 2 and 3 M GdnHCl, two partially folded intermediate states possessing flexible tertiary structures (revealed by fluorescence data) but compact secondary structures (as indicated by circular dichroism measurements) were identified
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Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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In brief, all FTIR experiments were performed on a Perkin-Elmer Spectrum 2000 spectrometer (Wellesley, MA) equipped with a dTGS detector
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Thus, the effective size of the monocation in compact DNA is expected to be closer to the size of free ion without hydration shell, where the sequence of the monocation radius is Li+ CONCLUSIONOur investigation highlights the inverse relation between DNA compaction potentials of monovalent cations in a crowded environment and the previously reported DNA binding properties of different alkali ions
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Protein compactness measured by fluorescence resonance energy transfer
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3; for details see Hsu and Bonvin, 2004), only the backbone configurational entropy starts to level off after ~4 ns (Fig
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To confirm these assignments, we examined a destabilized 4WJ ribozyme variant, in which G^sub 11^ is replaced by inosine (I)
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We note that the term "compact" is used in this article to denote the proximity of arms A and B, as described by the FRET efficiency for the current labeling scheme
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