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. The pD values were read directly from an Orion pH meter (Orion Research, Beverly, MA) and no corrections were made for isotope effects
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. (21) This, however, does not preclude the possibility that some receptors may ultimately be shown to perform both tethering and tickling (signal transduction) functions
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. 5 a, the transition temperature is nearly identical for samples with and without NDSB
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. The qualitative structural implication is, therefore, that the second CaM monomer in the dimeric complex is placed along this axis in such a way that the tips of the N- and C-terminal domains touch each other
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. Drawing out the seediness underlying the glitz, McManus's book did something that the others have failed to--subtly observed that the single-minded pursuit of money often exacts a heavy price
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- (On the form, the donor's name has been poorly redacted: the first word begins with "P" and a subsequent word starts with "M," suggesting that the source was the Pharmaceutical Research and Manufacturers Association)
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. The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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. Stock solutions of ANS were prepared in water by using a molar extinction coefficient of 6
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. Such a link between the instability to freeze-thawing and propensity to bind ANS in ice supports the notion that irreversible damage to freeze labile proteins, often resulting in enzyme inactivation and or protein aggregation, is likely to have originated from partial unfolding of the globular structure in ice
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. Crystal structure of the disulfide bond-deficient Azurin mutant C3A/ C26A
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. The Trp fluorescence of acid unfolded TTR peaks at 352 nm (data not shown) compared to 340 nm in the native protein, indicating unfolding
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