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. On the other hand, the EIC domain contains the PEP-binding region and the dimerization interface, as shown by the crystal structure of the EIC from Thermoanaerobacter tengcongensis (11)
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. DSC experiments were performed under a constant external pressure of 30 psi to avoid bubble formation and samples were healed at a constant scan rate of 60 K/h
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. Protein solutions (protein concentrations: 7 µg/ml to 1 mg/ml) were tilled into quartz-cuvettes with an optical pathlength of 1 cm (104F-QS, Hellma, Muehlheim, Germany)
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. For the drug uptake experiments, the oocyte was then immersed in an aqueous solution of 250 mM diminaze aceturate (Sigma) and inserted into the MR tube loaded with the same solution
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. Other MD studies have demonstrated that transient openings in the wall of the gorge, so-called back-doors or side-doors, provide putative alternate routes for traffic of substrate, products, and/or solvent (29,30)
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. A new and rapid colorimetric determination of acetylcholinesterase activity
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. Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution
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. DISCUSSIONThe influence of the chemical nature of a monocation on its DNA compaction ability is clearly demonstrated by the observation of the collapse of single DNA molecules by fluorescent microscopy
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. INTRODUCTIONMany diseases are caused by protein misfolding
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. The date acquisition card settings were chosen to give a time resolution 10-100 ps depending on the decay time of the fluorescent probe in question
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. 6; under these conditions, the protein self-association is minimal and the predominant apoA-2 form is a disulfide-linked dimer (Swaney and O'Brien, 1978; Donovan et al
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