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. 3), we used fluorescence quenching to further investigate the solvent accessibility of tryptophan residues upon unfolding
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. Evidence for the existence of an unfolding intermediate state for aminoacylase during denaturation in guanidine solutions
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. Both a greater affinity and a larger number of binding sites attest to a significantly greater perturbation of tertiary structure in the azurin mutants than in the WT
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. Using a high magnetic field and a home-built microscopy probe with large gradient field strengths, we achieved voxel sizes of (180 µm)^sup 3^
2005 adipex followup october post
. 5 mM KCl, 1 mM CaCl^sub 2^, 1 mM MgCl^sub 2^, 1 mM Na^sub 2^HPO^sub 4^, 5 mM HEPES, pH 7
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. ChEs provide a particularly suitable system for studying relationships between activity, ligand binding, unfolding, and thermal molecular fluctuations due to the following properties:1
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2005 adipex followup october post
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2005 adipex followup october post
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. Fluorescence resonance energy transfer (FRET) is a very informative technique for studying the tertiary folding of the hairpin ribozyme
2005 adipex followup october post
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. * The treatment component of the Problem-Gambling and Responsible-Gaming Strategy should be comprised of an integrated, community-based approach that includes the utilization of the best available treatment methodologies, alternative and flexible delivery models, the collection and dissemination of meaningful data on client attendances, treatment-counsellor hours and outcome monitoring based on longitudinal studies with sound client follow-up procedures
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. These results suggested that during ACYI thermal denaturation, the dominant events were the unfolding of the β-sheet (1638 cm^sup -1^) and the formation of the intermolecular β-sheet in aggregates (1617 cm^sup -1^)
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