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In experiments with the effectors (5 mM Mg^sup 2+^ and 2
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For example, at 20 µm ANS where binding sites are fully saturated in solution and the intensity has reached its maximum value, the corresponding intensity in ice is merely 3%-5% of the value in the liquid water
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The method was also successful with 102 eggs collected from Wakasa Bay (Table 4), which had been immediately fixed with 5% formaldehyde in seawater
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These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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