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. , at the tips of the N- and C-terminal domains, ii), close to the hinge region connecting the N- and C-terminal domains, iii), close to the hydrophobic pockets in the N- and C-terminal domains where the target binds (see Fig
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. DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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. The role of dynamics in enzyme activity
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. For example, the calculated ratio of k^sub cat^ (DmAChE/HuBChE) for hydrolysis of various substrates at 25°C is 1
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. The dominant elasticity results from distortions in the average particle configurations and is thought to be entropic in nature (70)
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. Indeed, several lines of evidence indicate that both pressure-induced and thermal denaturation of HuBChE are multistep processes that are initiated by penetration of water into the active-site gorge, leading to protein swelling and, eventually, unfolding (18,53,54)
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